Table 2-1. Precision (R.S.D. %) and accuracy (R.E. %) for MPAG in microsomal
incubations (six replicates per day for three days).
Concentration (pM) R.S.D. (%)a R.E. (%)
Nominal Measured (Mean) Intra-day Inter-day
2.50 2.70 5.6 8.9 8.0
7.50 7.40 3.9 5.1 -1.3
16.0 16.5 6.1 6.8 3.1
aEstimated using one-way ANOVA
Table 2-2. Assessment of extraction recovery, matrix effect, and stability of MPAG
analytical assay.
Nominal MPAG
Nominal MPAG Extraction Recovery a Matrix Effect b Stability C
Concentration
(poM) (%) (SD) (%) (SD) (%) (SD)
(PM)
2.5 83.3 88.0 105.3
16 91.6 87.3 109.7
50 (PG) 103.4 96.9 107.0
a Extraction recovery was calculated using the following formula: Recovery
(%) = [(mean raw peak area)pre ext. spike/(mean raw peak area)post ext. spike] x 100.
b Matrix effect was calculated using the following formula: Matrix effect (%) = [(mean
raw peak area)post ext. spike/(mean raw peak area)neat] x 100.
C Stability was calculated using the following formula: Stability (%) = [(mean raw peak
area)after 36 hours/(mean raw peak area)initial run] x 100.
PG = phenolthalein glucuronide (internal standard)