CHAPTER 5 CONCLUSIONS AND RECOMMENDATIONS Both treated and untreated filters exhibited high viable removal efficiency (> 99.996%) for bacterial spores in various environmental conditions with negligible variation in pressure drop. This great performance of test filters did not deteriorate over the experimental duration (i.e., 10 hr or 4 hr). Viability of spores collected on the filter was investigated by extracting them from the filter and presented as the survival fraction. A higher survival fraction on the untreated filter than that on the treated filter was reported at RT/LRH. However, the survival fraction of treated filters at RT/HRH and HT/HRH was similar to that of untreated filters tested at RT/LRH. Loss of iodine by sublimation and dissolution at HT and HRH was responsible for the indifference. As electret filter media, both treated and untreated filters presented high retention capability for negatively charged bacterial spores and thus reduced potential hazard from the release of spores from the media. For the viral aerosol filtration experiment, new filter media different from those used in the bacterial spore experiment were used. The iodine-treated filter presented high removal efficiency for viral aerosols with low breathing resistance (significantly lower pressure drop than the NIOSH regulation) under various environmental conditions. Both treated and untreated filters presented similar extracted fractions, indicating insignificant difference in the infectivity of viruses on both filters. This observation can be attributed to the shielding effect of MS2 aggregates on the filter and/or high retention capability of the filter due to electrostatic interaction between charged filter media and viral particles making any difference indistinguishable. Meanwhile, it demonstrates again high retention capability that can minimize reaerosolization and prevent air filters to be a potential source of microbial contamination. As an inactivation mechanism of the iodine-treated filter, transfer of a lethal dose of iodine from the