CHAPTER 3 METHODOLOGY TO DEVELOP A NUTRIENT INVENTORY AND PUBLIC WILLINGNESS TO PAY FOR WATER QUALITY IN THE ICHETUCKNEE SPRINGS SYSTEM Nutrient Inventory of the Ichetucknee Springshed Ichetucknee River To quantify the N mass-balance equation of the Ichetucknee springshed system, the total mass of Nitrate leaving the system via the River was needed. This was calculated by multiplying the mean concentration, by mass, of nitrate in the river water by the total flow in Liters-per-day Atmospheric Deposition Atmospheric deposition of nitrogen and other elements is a naturally occurring phenomenon that has been altered by human activities, specifically the burning of fossil fuels and the release of nitrogen- and sulphur-oxides into the atmosphere. It is then deposited on the land surface by rainfall and dry deposition. The National Atmospheric Deposition Program (NADP) is a cooperative effort between the State Agricultural Experiment Stations, U.S. Geological Survey, U.S. Department of Agriculture, and numerous other governmental and private entities which monitors atmospheric deposition of nitrogen and other substances. The estimate for nitrate loading contributed by atmospheric deposition were obtained using data collected by the NADP/NTN station located in Bradford Forest (Bradford County, Florida; ca. 50km from Ichetucknee) (NADP, 2006) and multiplying by the area of the springshed. Lake City's Waste Water Treatment Facility (WWTF) Sprayfield Lake City is a town of roughly 14,000, located in Columbia County. The city's effluent irrigation spray-field system was completed in 1987, sending secondary effluent from the St. Margaret Road Water Reclamation Facility through 4 miles of pipeline to the effluent irrigation site. Effluent is applied to the treatment fields through an irrigation system consisting of 70