Table 3-1. Infection efficiencies ofE. cobi TG1 containing various helper plasmids with
phagemid particles. Bacterial strains were transduced with anti-BSA (a-BSA) and
Vc86 phagemid particles at a MOI of 0. 1, and transduced bacteria were enumerated.
Infection efficiency is the number of transductions into a bacterial strain divided by
the number of transductions into E. cobi TG1 and is represented as a percentage.
Infection Infection Average
efficiency efficiency infection
Transductions with Transductions with Vc86 efficiency
Strain with a-BSA a-BSA (%) with Vc86 (%) (%)
E. cobi TG1 1.2 x 106 100 3.6 x 106 100 100
E. cobi TG1 9.3 x 102 0.1 6.9 x 103 0.3 0.2
(M13cp-sm)
E. cobi TG1 8.3 x 102 0.1 9.6 x 103 0.3 0.2
(M13cp-lg)
E. cobi TG1 7.9 x 104 7 4.8 x 105 13 10
(M13cp-CT-sm)
E. cobi TG1 <1.3 x 102 <0.01 <1.3 x 102 <0.01 <0.01
(M13cp-CT-lg)
E. cobi TG1 2.1 x 106 175 3.6 x 106 97 136
(M13cp-dg3-sm)
E. cobi TG1 <1.3 x 102 <0.01 <1.3 x 102 <0.01 <0.01
(M13cp-dg3-lg)
Table 3-2. Infection efficiencies of E cobi TG1 (M13cp-dg3-sm) with phagemid particles.
Bacterial strains were transduced with phagemid particles at a MOI of 0. 1, and
transduced bacteria were enumerated. Infection efficiency is the number of
transductions with a phagemid into a bacterial strain divided by the number of
transductions with the same phagemid into E. cobi TGl.
Infection
Strain Phagemid Transductions efficiency (%)
E. cobi TG1 Vc86 5.3 x 106 100
E. cobi TG1 (M13cp-dg3-sm) Vc86 4.9 x 106 92
E. cobi TG1 Clone 18 2.4 x 106 100
E. cobi TG1 (M13cp-dg3-sm) Clone 18 2.7 x 106 113
E. cobi TG1 a-AV20N3 1.5 x 106 100
E. cobi TG1 (M13cp-dg3-sm) a-AV20N3 1.8 x 106 120