Reproductive lifespan was calculated as the time between first and last eggs laid. Clutch size was
not quantified because oviposition in C. morosus occurs continuously throughout the
reproductive lifespan rather than in discrete clutches. After death, each female was dissected, and
the number of ovarioles in both ovaries was determined. The numbers of fully chorionated eggs
and non-chorionated eggs in terminal follicles and in the oviducts were counted. Although I
intended to evaluate hatch success, egg viability was inexplicably low in this study, particularly
compared to the expected hatchability of nearly 100% (Brock 2000).
Statistical Analyses
Analysis of variance (ANOVA) was used to test for differences among treatment groups.
Data were first tested for normality (Shapiro-Wilk test) and homogeneity of variances (Levene's
test) and transformed, if necessary, using a natural log, reciprocal, square root, square, reciprocal
square, or reciprocal square root transformation. Pairwise comparisons were evaluated using
Tukey's Honestly Significant Difference post hoc test (if variances were homogeneous) or
Tamhane's T2 post hoc test (if variances were not homogeneous). If transformation did not
normalize data, they were analyzed using a Kruskal-Wallis test, and pairwise comparisons were
evaluated using Mann-Whitney U tests with a set at 0.005 to account for the number of
comparisons tested (ten). Reproductive output was analyzed using ANOVA as described above
and also using analysis of covariance with body mass at first oviposition as the covariate to test
for size-independent differences in allocation to reproduction. Other covariates (including length
at first oviposition, body mass at adult molt, and length at adult molt) could not be used because
of significant interactions between these variables and treatment group.
Stepwise linear regression was used to determine the factors that best explained variance in
reproductive output. For this analysis, the dependent variable was the cumulative fecundity of
each female. The independent variables tested were mass-specific intake and growth rates during