using the LOGISTIC procedure of SAS. The statistical model for each experiment was the same

as described above. The data are reported as the actual percentage.

 Embryo stage in both experiments was analyzed using both the LOGISTIC and

CATMOD procedures of SAS. The statistical models for each experiment were the same as

described above. The statistical values obtained after analysis with LOGISTIC and CATMOD

were similar and only statistical inferences from the LOGISTIC analysis are reported. Data are

reported as the actual percentage.

 Results

Experiment l(Group Transfer of Embryos)

Embryo development in vitro

 Addition of IGF-1 to culture increased (P < 0.05) cleavage rate on day 3 (Control 80.9

+ 0.8% vs. IGF-1 84.0 + 0.8%). However, there was no effect of IGF-1 on the proportion of

oocytes that developed to the blastocyst stage (Control 27.3 1.6% vs. IGF-1 28.7 1.6%)

or advanced blastocyst stages (Control 14.9 0.6% vs. IGF-1 14.7 0.6%) on day 8 after

insemination.

Embryo recovery and development at day 14

 Supplementation of culture medium with IGF-1 did not affect the proportion of embryos

recovered at day 14 (Table 3-1). Among embryos recovered, there was also no effect of IGF-1

on embryo length, IFN-T secretion, or the proportion of embryos with a visible embryonic disc

(Table 3-1). In addition, treatment with IGF-1 did not affect embryo stage at day 14 (Table 3-2).

 Recovery rate and embryo length were affected by flush type (i.e., slaughter vs. live

animal). Recovery rate tended to be greater (P < 0.06) for embryos that were recovered after

slaughter than for embryos collected by non-surgical procedures (37.8 6.2% vs. 21.8 4.1%,

respectively). In addition, embryo length was greater (P < 0.01) for embryos recovered