Table 2-1. Primers used for RT-PCR.
 Annealing temp
 Genes Primer sequences and positionsC) x cycle number Fragment EMBL accession no.
 and embryo size (bp)
 equivalent
 Glucose transporter 1(Glutl) 5'primer: (894-914) = CAG GAG ATG AAG 59 C x 32 257 M60448


SLC2A1


Glucose transporter 3 (Glut3)
SLC2A3



Glucose transporter 8 (Glut8)
SLC2A8


Bax


BCL-xL (Bcl)




HSP70.1 (Hsp70)


GAG GAG AGC
3'primer: (1131-1151) = CAC AAA TAG
CGA CAC GAC AGT

5'primer: (1095-1118) = CCT TGG AGG
GAT GGC TTT TTG TTC
3'primer: = CGT GGC TGA GGG GAA
GAG CAG TCC

5'primer: (711-730) = CCT CGC TTC CTG
CTG TCT CA
3'primer: (935-954) = CCT CCT CAA AGA
TGG TCT CC

5'primer: (227-249) = TGC AGA GGA TGA
TCG CAG CTG TG
3'primer: (402-424) = CCA ATG TCC AGC
CCA TGA TGG TC

5'primer: (197-221) = ATG GAG CCA CTG
GCC ACA GCA GAA G
3'primer: (479-503) = GTT GCG ATC CGA
CTC ACC AAT ACC T

5'primer: (844-864) = GGG GAG GAC TTC
GAC AAC AGG
3'primer: (1068-1088) = CGG AAC AGG
TCG GAG CAC AGC


0.05


59 C x 32
 0.1



58 C x 34
 0.2


259




244


600C x 32
 0.1


600C x 32


600C x 32


307


245


NM 174603




AY208940.1


NM 173894.1


NM 001077486


NM 174550.1