For development of MFLs, it is necessary to identify a benchmark period from which flow deviations may be evaluated. Often changing flow trends are presumed to be the result of anthropogenic stresses on a system. This is possibly because variation in flow is frequently assumed to be the result of random independently and identically distributed random variables such as rainfall in flood risk analysis (Olsen et al. 1999). However, the effect of multidecadal oscillation on river flow patterns must also be recognized as natural climatic variations. As Enfield et al. (2001) observe, the Atlantic Multi-decadal Oscillation has a pronounced effect on rainfall in the continental United States and wet-season rainfall in peninsular Florida is negatively correlated with the Atlantic Multi-decadal Oscillation (McCabe and Wolock 2002). Kelly (2004) examined stream flows in Florida and identified a step-trend similar in timing to the step-trend describing the AMO (Enfield et al. 2001). This resulted in the identification of two benchmark periods, one from 1940-1969 and one from 1970-1999, which are characterized by relatively higher and lower wet season river flows in peninsular Florida, respectively. For determination of minimum flows, records from both benchmark periods are analyzed, and used to develop percent-of-flow reduction criteria. Minimum flows are determined by calculating the percent flow reduction for each benchmark period that would result in no more than a 15% reduction in habitat. For the Block 3 period, this means identifying the flow reduction that would result in no more than a 15% loss in the number of days of days that floodplain features are inundated by the river, during each of the two benchmark periods and utilizing the more limiting of the two. In establishing minimum flows, the District defines a 15% change in habitat availability as a criterion for identifying significant harm, or unacceptable change. For the high-flow period or Block 3, this change is expressed as a temporal difference in the number of days specific water