be considered quantitative, making the imprinting possible. The imprinting strategy is shown in

Figure 7-2.

Table 7-1. Loading compositions of the miniemulsions.
 Sampl EGDM MAAAmitriptyline
 hydrochloride
MIP 1 600 mg (3.03 mmol) N/A N/A
MIP2 550 mg (2.77 mmol) 50 mg (0.581 mmol) N/A
MIP3 500 mg (2.52 mmol) 100 mg (1.162 mmol) N/A
MIP4 600 mg (3.03 mmol) N/A 50 mg (0.159 mmol)
MIP5 550 mg (2.77 mmol) 50 mg (0.581 mmol) 50 mg (0.159 mmol)
MIP6 500 mg (2.52 mmol) 100 mg (1.162 mol) 50 mg (0.159 mmol)



 ~C Template Crosslinker Radical initiator
 SBinding monomer Porogen Stabilizing surfactant




 ~ r 311 crosslinking
 *.~ g / template, porogen
 / 4.1 _/ and surfactant removal





Figure 7-2. The molecular imprinting strategy in miniemulsion polymerization.

 The efficiency of the cross-linking reaction was demonstrated by infra-red (FTIR)

spectroscopy. Figure 7-3 shows the FTIR spectra of MIP1 and MIP3, and the FTIR spectrum of

pure EGDMA before reaction is also included for comparison. The absorbances of the peaks at

1640 cm-l (stretching vibration frequency of the alkenyl C=C double bonds) and at 3100 cml

(stretching vibration frequency of the alkenyl C-H single bonds) almost completely vanished for

MIP1 and MIP3, indicating that the doubles bonds of EGDMA and MAA were successfully