Abstract of Thesis Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Master of Science USING MULTILEVEL SAMPLERS TO ASSESS ETHANOL FLUSHING AND ENHANCED BIOREMEDIATION AT FORMER SAGES DRYCLEANERS By Gordon Hitchings Brown August 2006 Chair: Michael D. Annable Cochair: James W. Jawitz Major Department: Environmental Engineering Sciences As a result of dry cleaning operations, there are hundreds of sites where the subsurface aquifer has been contaminated by perchloroethylene (PCE), the dry cleaning agent. One of the more challenging problems facing environmental scientists and engineers today is locating PCE source areas and cleaning up these sites to reduce the risk of contaminated groundwater reaching water supplies. The high specific gravity, low solubility, and recalcitrance make PCE highly persistent in the environment. Without some remedial action, many of these sites would serve as a source of dissolved groundwater contamination for decades. The Sages former drycleaning site in Jacksonville, FL, was the test ground for a pilot scale in-situ alcohol flushing test in 1998. The sandy aquifer was contaminated with PCE, found as a separate phase in discrete layers in the sub surface. A network of multilevel sampling wells (MLS) was installed in the source area to collect liquid samples before, during and for six years after the pilot test. The depth level sampling of MLS allows three dimensional spatial analyses. Multilevel samples were able to determine the initial and post-remedial PCE architecture at the site. This information will help the site manager