50
 AD

 40


 30


 20 UDP





 0:00 5:00 10:00 15:00 20:00 25:00 30:00min

Figure 6-6. HPLC chromatogram of UDP, UDP-galacturonic acid (UDPGTA), and
 UJDPGA standards.



 Results

 Several catfish liver and whole intestines were analyzed for UDPGA content using

this method. The livers had been put in plastic tubes (with no buffer) immediately

following sacrifice, and stored at -800C. A paired t-test revealed that mean concentrations

in liver were significantly higher than in intestine (p=0.008, Table 6-3 and Figure 6-7).


Replicates showed less than 25% SD except for two fish (AT17 and AT18). Interestingly,

the intestinal UDPGA values for one fish (AT17) were comparable to the hepatic

UDPGA values of another fish (GS39).