A


 1 min boiling in 0 .25M buffer







 20








 0:00 5:00 10:00 15:00 20:00 25:00 30:00 35:00 40:00mi


 B


 1 min boiling in 0 .30M buf fer, pH 4. 3



 30



 20



 10




 0:00 5:00 10:00 15:00 20:00 25:00 30:00 35:00 40:00 mi



Figure 6-3. Effect of boiling liver tissue for 1 minute in two different concentrations of
 buffer. A, 0.25 M H2PO4, pH 3.4; B, 0.30 M H2PO4, pH 4.3




HPLC

 A mobile phase consisting of 0.3 M NH4H2PO4 in water (pH 3.1) was initially

tried. UDPGA standards eluted at 16 min. However, this elution time was found to be