The sequence of the DNA represented by these clones was compared to the UGT

sequence deduced from the partial sequences of the various overlapping clones and

amplicons (Table 5-13). There were minimal variations (99% homology), with the

exception of a triple base change from AAA to TTT in all the clones at position 648.



Table 5-13. Results of ClustalW multiple sequence alignment analysis of the cloned
 UGTs and the original livUGTn


SeqA Name


Len(nt) SeqB Name


Len(nt)

1782
1799
1798
1799
1798
1798


Score

99
99
99
99
99
99


Score

99
99
99
99
99
99


livUGTn
livUGTn
livUGTn
UTR1
UTR1
UTR2


1566
1566
1566
1782
1782
1799


UTR1
UTR2
UTR3
UTR2
UTR3
UTR3


SeqA Name


Len(nt) SeqB Name Len(nt)


UGT 1
UGT 1
UGT 1
UGT2
UGT2
UGT3


1569
1569
1569
1569
1569
1569


UGT2
UGT3
livUGTn
UGT3
livUGTn
livUGTn


1569
1569
1566
1569
1566
1566


For full-sequences of these full-length UGT clones, refer to Appendix B.