Protein sequence analysis
 a.1iv/intUGTp. Further confirmation that the predicted livUGTp is a UGT came

from a conserved domain search (Figure 5-16). The sequence was 100% aligned with

UDPGT, and it was also closely related to glycosyltransferases and N-acetylglucosamine

transferases.



 100 200 300 400 521
 I I I UDP I


 I hwjDomain Relatives

 Score E
 PSSMs produc~n signfcant alinments (blts) value

 an1|CDD|24386 pfarnOO201, UDPGT, UDP-glucoronosy1 and UDP-glucosy1 transfers. 534 Be-153
 an1|CDD|26194 pfarnO4101, Glyco_tran_28_C, Glycosyltransferase farmly 28 C-te 44 6 3e-05
 gn1|CDD|11529 COG1819, COG1819, Glycosyl transferases, related to UDP-glucur 85 9 le-17
 an1|CDD110576 COGO707, MurG, UDP-N-acetylgucosarnine:LPS N-acetylglucosatnine 43 B 6e-05


Figure 5-16. Results of NCBI conserved domain search

 PSSM: position-specific scoring matrix


 The protein displayed several areas of marked hydrophobicity, particularly at the C-

terminal end, which presumably corresponds to the transmembrane region, which is

followed by the positively charged cytoplasmic tail (Figure 5-17). Based upon the

method developed by Kolaskar and Tongaonkar (1990), the Emboss program antigenic

identified several potential antigenic sites. Maximum score position is denoted by the

residues in bold and underlined format (Table 5-11).


41HI~ilia

 11urG


~ilili~