1.punctatus
 RnorvegicuslA~2
 RnorvegicuslA6
 RnorvegicuslA1
F.catusUGT1A1
H.sapiensUGT1A1


F.catus UGT1A1
H.sapiensUGT1A1
R.norvegicuslA1
R.norvegicuslA2
R.norvegicuslA6
I.punctatus livUGTp


F.catus UGT1A1
H.sapiensUGT1A1
R.norvegicuslA1
R.norvegicuslA2
R.norvegicuslA6
I.punctatus livUGTp


F.catus UGT1A1
H.sapiensUGT1A1
R.norvegicuslA1
R.norvegicuslA2
R.norvegicuslA6
I.punctatus livUGTp


F.catus UGT1A1
H.sapiensUGT1A1
R.norvegicuslA1
R.norvegicuslA2
R.norvegicuslA6
I.punctatus livUGTp


QKDVTVQDLMGSASVWLFRSDFVKDYSRP IMPNMVFIGGINCAGKNPLSQEFEAYVNASG
QREVTVQDLLS SASVWJLFRS DFVKDYPRP IMPNMVFVGG INCLHQNP LS QEFEAY INAS G
QKEVTVKDLLSPAS IWLMRNDFVKDYPRP IMPNMVFIGGINCLQKKALSQEFEAYVNASG
QREMSLVEVLSHASVWLFRGDFVFDYPRP IMPNMVFIGGINCVI KKPLSQEFEAYVNASG
QREVS LVEVLS HASVWJLFRGD FVFDYPRPVMPNMVF IGG INCV IKKP LS QEFEAYVNAS G
KKDVTFRDVLGHAAIWLYRYDFTFEYPRPVMPNAVRIGGINAKPADEFGS


EHGIVVFSLGSMVSAI PKEKAME IADALGKI PQTVLWRYTGTPPPNLAKNTI LVKWLPQN
EHGIVVFS LGSMVS E IPE KKAMA IADALGK IPQTVLWRYTGTRP SNLANNT ILVKWLPQN
EHGIVVFS LGSMVS E IPE KKAME IAEALGR IPQTVLWRYTGTRP SNLAKNT ILVKWLPQN
EHGIVVFS LGSMVS E IPE KKAME IAEALGR IPQTVLWRYTGTRP SNLAKNT ILVKWLPQN
EHGIVVFS LGSMVS E IPE KKAME IAEALGR IPQTVLWRYTGTRP SNLAKNT ILVKWLPQN
DHGF IVFTLGS FVS ELPE FKARE FFEAFRQ IPQRVLWRYTGV I PKD IPENVKVMKWLPQN


DLLGHPKARAFITHSGSHGIYEGI CNGVPMVMLPLFGDQMDNAKRMETRGAGLTLNVLEM
DLLGH PMTRAF ITHAGS HGVYE S ICNGVPMVMMP LFGDQMDNAKRMETKGAGVTLNVLEM
DLLGHPKARAFITHSGSHGIYEGI CNGVPMVMMPLFGDQMDNAKRMETRGAGVTLNVLEM
DLLGHPKARAFITHSGSHGIYEGI CNGVPMVMMPLFGDQMDNAKRMETRGAGVTLNVLEM
DLLGHPKARAFITHSGSHGIYEGI CNGVPMVMMPLFGDQMDNAKRMETRGAGVTLNVLEM
DLLAHPKAKVFITHGGAHGIYEGI CNGVPMVMI PLFGDQVDNVLRMVLRGVAESLTMFDL
***.* we .. was www*ws wwwwwwww*wwwwww*s we .w .w
---- UDPGA binding site ----------------->
TSEDLANGLKAVINDKSYKENIMRLSSLHKDRP IEPLDLAVFWVEFVMRHKGAPHLRPAA
TS EDLENALKAV INDKS YKEN IMRLS SLH KDRPVE PLDLAVFWVE FVMRH KGAPH LRPAA
TADDLENALKTV INNKS YKEN IMRLS SLH KDRP I EPLDLAVFWVEYVMRH KGAPH LRPAA
TADDLENALKTV INNKS YKEN IMRLS SLH KDRP I EPLDLAVFWVEYVMRH KGAPH LRPAA
TADDLENALKTV INNKS YKEN IMRLS SLH KDRP I EPLDLAVFWVEYVMRH KGAPH LRPAA
TSEQLLGALRKVLNNKRYKEKITQLSLIHKDRP IEPLDLAVFWTEFVMRHGSAEHLRPAA


F.catus UGT1A1
H.sapiensUGT1A:
R.norvegicuslA
R.norveaciuslA:


4-- TM region -9


Figure 5-13. (continued)


_1ivUlGTp


Figure 5-14. Phylogram for I.punctatus liv/intUGTp and selected mammalian UGT
 proteins


 b. Distinct partial sequence found in intestine (I35R_C). The partial nucleotide


sequence of I35RC (combined overlapping sequences of I35R and I35R PCR) was


subjected to a blastN search (Table 5-10) and shown to have homology with other UGTs


and unknown fish cDNAs. Multiple sequence alignment showed that livUGTn and I4_3R


are similar with respect to each other, while I3 5R was markedly different (Figure 5-15).