Viw1 =:.a-E-,-t- ; v Recl raw Si xFrvanes
I I I I I
I II I I I I


I I I I I I
I I I I
I I


UGTs and other unidentified proteins of the same species (Figure 5-12). The livUGTp

sequence was also aligned with mammalian UGTs (Figures 5-13 and 5-14).


 livUGTn


Frame from to Length
+1 0 112..1146 1035
+1 01150..1680 531
-2 o 147.. 575 429
 -1 0 421.. 846 426
 +2 o 908..1150 243
 -1 0 163.. 306 144
 +2 01565..1696 132
 +2 o 788.. 901 114
 +3 o 3.. 104 102


Figure 5-9. Identification of open reading frame using ORF Finder


1
51
101
151
201
251
301
351
401
451
501
551
601


SSKICFL*T*
SHWLSMKILV
LDESMNKLKE
NLREEHFDLM
PPSYVPRFFT
KKDVTFRDVL
DLEEFVDGSG
GVIPKDIPEN
VMIPLFGDQV
KITQLSLIHK
LDVIAFLLLV
GAVFN*RRWF
DVTKKKK


GMLRARVPGL
LCSFLGPVEG
IHGSDAYVAR
FTNMQVKGCE
VYFLRGLPCG
ESILCKIFFS
MPNAVRIGGI
AREFFEAFRQ
FITHGGAHGI
TSEQLLGALR
GSAEHLRPAA
RRAQKSKKE*
QFNIQGCQQL


FFSHSSSFFF
GKVLVMPVDG
SFKVPYTKAE
ALLYNEPLMQ
LDLEAAQCPS
SFDELTSRYL
NCAKKNPLPA
IPQRVLWRYT
YEGICNGVPM
KVLNNKRYKE
HHLNWVQYHS
NGQ*MIRNGF
WFSHCSYGCY


LET FKLKMPR
EELSRRGHEM
GITKAPRISD
LTDPFLPCGP
GNTDVMTFSQ
GHAAIWLYRY
DHGFIVFTLG
VKVMKWLPQN
DNVLRMVLRG
DRPIEPLDLA
LSTVVFIAVK
IGVMSYCENL


LLAALCLQIY
VVLVPETSVL
LLENIIGLLS
IIAEAFSLPA
RVKNVLMTGF
DFTFEYPRPV
SFVSELPEFK
DLLAHPKAKV
VAE S LTMFDL
VFWTEFVMRH
TCALCFRKCF
K*L*CSHHHV


Figure 5-10. Predicted protein sequence liv/intUGTp from liv/intUGTn

 indicate stop codons; highlighted residues indicate start and stop residues,
 determined after comparison with known UGT sequences, see Figure 5-11
 below)