Table 5-7. Results of blastn search for livUGTn (and intUGTn)
 Score E
Sequences producing significant alignments: (Bits) Value

gi|563246 17|emb|CR646752.3|ICNSOEYO6 Tetraodon nigroviridis full-length cDNA 97.6 le-16
gi|56242288|emb|CR644097.2|CNSOEVYF Tetraodon nigroviridis full-length cDNA 97.6 le-16
gi| 34850459|dbj|IAB12013 3.1| Pleuronectes yokohamae UGT1B2 mRNA, complete cds 75.8 5e-10
gi|71679708|gb|BC100055.1| Danio rerio cDNA clone IMAGE:7284571, partial cds 71.9 7e-09
gi|68369305|reflXM_682293.1| PREDICTED: Danio rerio similar to UGT 1, mRNA 71.9 7e-09
gi|68369293|reflXM_681739.1| PREDICTED: Danio rerio similar to UGT1, mRNA 71.9 7e-09
gi|465 18141|emb|BX005348.9| Zebrafish DNA sequence from clone, complete sequence 71.9 7e-09
gi|460165 16|emb|BX323 548.11| Zebrafish DNA sequence from clone, complete sequence 71.9 7e-09
gi|6537143|gb|AF104339.1|AF104339 Maacacafascicularis UGT1A01 mRNA comp. cds 63.9 2e-06
gi|47087384|reflNM_213422.1| Danio rerio zgc:66393 (zgc:66393), mRNA, complete cds 60.0 3e-05
gi|33416924|gb|BCO55635.1| Danio rerio zgc:66393, mRNA (cDNA) complete cds 60.0 3e-05
gi|50370246|gb|BCO75892.1| Danio rerio zgc:66393, mRNA (cDNA) complete cds 60.0 3e-05
gi|62531208|gb|BCO93347.1| Danio rerio zgc:66393, mRNA (cDNA) complete cds 60.0 3e-05
gi|3225 1578|emb|AL954329.7| Zebrafish DNA sequence from clone, complete sequence 60.0 3e-05
gi|81097721|gb|BC 109404.1| Danio rerio zgc:123097, mRNA (cDNA) complete cds 60.0 3e-05
gi|82658295|ref]NM_00010374281| Danio rerio zgc:123097 (zgc:1230), mRNA 60.0 3e-05
gi|507501 30|reflXM_421883.1| PREDICTED: Gallus gallus similar to UGT, mRNA 58.0 le-04
gi|89572711|lgb|AC161471.3| Gallus gallus BAC clone CH261-21B3, complete sequence 58.0 le-04
gi|46425671|lemb|BX931804.2| Gallus gallus finished cDNA, clone ChEST795fl9 58.0 le-04



Table 5-8. Promoter prediction. Predicted transcription start is shown in larger font.

Start End Score Promoter Sequence

97 147 0 .99 AA TTAGAAACTT TTAAGCTAAA AATGCCTCGT CTTCTTGCAGCTCT

480 530 0.98 GAAAGGATGCGAGGCGCTGCTGTATAACGAGCCTCTGATGAG CTC

1425 1475 0.93 GATAACACAGCTGTCTTTGATCCATAAAGACCGTCCGATCG CGTG

1710 17 60 0 .95 CAGGAAT GGATTT GGT GCCGT CTTTAATTAACGCCGAT GGT TTAT CGGCG

Bold sequence indicates most likely promoter


 The open reading frame (ORF) was identified by translating the sequence data of

all possible frames (Figure 5-9) and choosing the one that showed the least stop codons

(Frame +1). The translated sequence (Figure 5-10) was then subjected to a blastp search

with other protein sequences in GenBank (Table 5-9), followed by alignment of these

sequences. In this way, the untranslated regions (UTRs) were also identified (Figure 5-

11). The catfish liver sequence was found to have the best similarity with Danio rerio