100bp ladder L8 L7 L6 L5 L4 L3 L2 L1 1kb ladder





 ~3,000


 600
 S300








Figure 5-4. Product of ecoRI digest of purified plasmids containing liver inserts L1-L8




 The DNA sequences obtained are detailed in Appendix A. The results of the

BLASTn search (best five sequences for each insert) are summarized below (Table 5-3).

Very good matches were obtained with the Tetraodon nigroviridis cDNA, as well as

Pleuronectes yokohamnae UGT I B2, several Danio rerio sequences and

Strongylocentrotus purpuratus (sea-urchin) UGT2B sequences. There was also a good

similarity between the longer insert and the mammalian UGTIA sequences.

 Better matches were obtained with the longer cDNA insert obtained from the liver

(95 sequences with score >50) than with the shorter insert from liver or intestine (9

sequences with score >50).