0 1 2 5 10 20 50 75 100
 E-M PCP


Figure 3-8. Autoradiogram showing reverse-phase TLC separation of sulfonation
 products from the study of PCP kinetics.

 The arrow indicates the sulfate conjugate of PCP, while other bands represent
 unidentified sulfate conjugates formed from endobiotics or other xenobiotics
 in polar bear liver cytosol.


 Discussion

 The sulfonation of hydroxylated metabolites of benzo[a]pyrene has been reported

in various species, including fish (James et al., 2001) and humans (Wang et al., 2004).

Benzo[a]pyrene-3 -glucuronide has been shown to be produced by fish (James et al.,

1997), rats (Lilienblum et al., 1987) and humans (Wang et al., 2004). There are,

however, few studies investigating the kinetics of these conjugation reactions.

Glucuronidation of 3-OH-B[a]P was more efficient in polar bear liver than in human liver

or catfish intestine. On the other hand, the efficiency of sulfonation was similar to that

shown in human liver but around three times less than in catfish intestine (Wang et al.,