substrate blank, the fact remained that we were apparently looking at the only instance

ever reported of a successful sulfonation of an acyclic tertiary alcohol.








 *#4@










 PC P100 CO C100 HO H100


Figure 3-6. Autoradiogram showing the reverse-phase TLC separation of sulfonation
 products from incubations with TCPM using polar bear (P), channel catfish
 (C), and human (H) liver cytosol in the absence of (0), and presence of 100
 CLM TCPM (100).

 The arrow indicates the sulfate conjugate of the substrate, while other bands
 represent unidentified sulfate conjugates formed from endobiotics or other
 xenobiotics in liver cytosol.


 Thus, additional experiments were performed to verify the identity of this

conjugate. The purity of the TCPM was tested in the event that the additional band was

due to an impurity in the substrate. However, the substrate used was found to be free of

contaminants by HPLC (C18 reverse phase column, with detection at 268 and 220 nm,

using 90% methanol in water and a flow rate of 1 mL/min). A single peak was recorded