0 20 50 '100 200 300
 IgM OHMXC


Figure 3-5. Autoradiogram showing the reverse-phase TLC separation of sulfonation
 products of OHMXC.

 Incubations were carried out with the indicated concentrations of OHMXC.
 The arrow indicates the sulfate conjugate of the OHMXC, while other bands
 represent unidentified sulfate conjugates formed from endobiotics or other
 xenobiotics in polar bear liver cytosol.


 The total TCPM sulfate conjugate production formed after 5 minutes under initial

rate conditions did not exceed 30 pmol. TLC, followed by autoradiography, was thus

used to distinguish the TCPM-sulfate band (Rf 0.54) from other sulfate conjugates (Rf

0.05 and 0.72) originating from compounds in the polar bear liver cytosol (Figure 3-6).

 The data obtained followed hyperbolic kinetics (Table 3-2). Even though the TLC

from the kinetic experiments showed a TCPM concentration-dependent increase of the

band corresponding to the purported TCPM-sulfate, and this band was absent in the