LIST OF TABLES


Table pg

2-1 Expression of human UGT mRNA in various tissues ................. ........... ..........9

2-2 Tissue distribution of SULTs (cDNA and mRNA) in humans.............. ...... ........._12

3-1 Estimated kinetic parameters (Mean + SD) for (a) sulfonation and (b)
 glucuronidation of 3-OH-B[a]P by polar bear liver cytosol and microsomes. ........24

3-2 Kinetic parameters (Mean + SD) for the sulfonation of various xenobiotics by
 polar bear liver cytosol, listed in order of decreasing enzymatic efficiency. ...........27

4-1 Estimated kinetic parameters (mean & S.D.) for the co-substrate UDPGA in the
 glucuronidation of three different OH-PCBs. ...........__......_ ................44

4-2 Kinetic parameters (Mean & S.D.) for the glucuronidation of 4-OHBP and OH-
 PCBs. ........... ..... .. ...............48....

4-3 Comparison of the estimated kinetic parameters for OH-PCB glucuronidation in
 catfish liver and proximal intestine .............. ...............48....

4-4 Comparison of kinetic parameters (Mean & SEM) for the glucuronidation of
 OH- PCBs grouped according to the number of chlorine atoms flanking the
 phenolic group ........._.__...... ..__ ...............49....

4-5 Results of regression analysis performed in order to investigate the relationship
 between the glucuronidation of OH-PCBs by catfish proximal intestine and liver
 and various estimated physical parameters. ............. ...............52.....

5-1 5' 3' Sequences of degenerate primers chosen. ......___ ... ...... ..............66

5-2 Primer pairs chosen, showing annealing temperature and estimated amplicon
 length ................. ...............67.................

5-3 Results of BLASTn search of cloned putative partial UGT sequences ........._........78

5-4 Gene-specific primers used in initial 5'RLM-RACE study. ................ ................82

5-5 Gene-specific primers used in succeeding RLM-RACE study ............... .... ............83

5-6 Primers used for amplifying liver and intestinal UGT gene .............. ..................87