Table A- 2. Oligo Tucleotide description.
Primer Cone.l Sense2 Description Tm3
 TB1 0.892 S addition of histidine-epitope tag (HHHHHH) to the N- 85
 TB2 0.606 A terminus of the b subunit; +Sphl
 TB3 0.329 S addition of HA-epitope tag (YPYDVPDYA) to the N- 85
 TB4 0.414 A terminus of the b subunit; +Ndel
 TB5 2.846 S addition of Sphl site 3' of the b subunit for cloning 78
 TB6 2.236 A purposes; + Sphl
 TB7 1.95 1 S mutation of Sphl site 3' of the b subunit and addition 78
 TB8 1.593 A of a favorable "ATG" prior to next gene; -Sphl
 TB10 0.308 S 6cysl40 ser mutation; +EcoRI; -Clal 80
 TB11 0.650 A
 TBl2 0.977 S bcys20 ser mutation; -SnaBI 79
 TBl3 1.538 A
 TBl4 1.151 S bgly43 cys mutation; -Bspl285I 77
 TBl5 1.446 A
 TBl6 1.100 S bser84 cys mutation; -Xbal 75
 TBl7 1.290 A
 TBl8 1.366 S 6cys64 ser mutation 82
 TBl9 1.242 A
 TB20 2.684 S sqences b in the sense (not a good one) 75
 TB21 2.422 S seuences 6 in the sense direction 81
 TB23 1.380 S acys90 ser mutation; +BamHI 77
 TB24 1.640 A
 TB25 5.016 S seuences a in the sense direction 78
 TB27 0.886 S mutations to create Shine Delgarno sequence upstream 76
 TB28 1.23 5 A of the 2nd b gene in "mix" plasmids
 TB29 5.279 A sqences b in the inverse complement direction 79
 TB30 4.334 S deletion of residues 1-24 of b subunit to create "bsol" 85
 TB31 4.608 A
 TB32 8.301 S addition of histidine-epitope tag (HHHHHHHHHH) to 78
 TB33 7.618 A the N-terminus of the "bsol" subunit
 TB36 0.439 S mutations to create a better Shine Delgarno sequence 78
 TB37 0.477 A upstream of the 2nd b gene in "mix" plasmids
 TB38 12.33 S addition of V5-epitope tag (GKPIPNPLLGLDST) to 79
 TB39 9.310 A the C-terminus of the b subunit; +SacI
TB40- 4.522 S deletion of last 4 amino acids at the C-terminus of the 76
TB41- 4.891 A b subunit; +HindlIII
TB40+ 1.109 S duplication of last 4 amino acids at the C-terminus of 78
TB41+ 0.983 A the b subunit; +SacI
SConcer tration of stock 01 gonucleotide in Clg/CIl, stored in the -200C.
2 Direction of the primer: S, sense; A, antisense.
3 Melting temperature in ag.