Mu tan ts G rowt h

 b+153-156
 A153-156-his

 b+1 43-1 46 +
 b +++
 A143-146

 bA124-125

 +124-125

 b+124-130 +

 b+1 02-1 05 +

 6b102-105

 g *b+90-93
 *b1~ +++
 B Je+73-76
 b+59-62

 *tether region
 insertions
 and deletions

 barg24ala,thr64ala,glnl 04ala


 .bthr64ala +
 barg24ala

Figure 5-5. Mutations constructed throughout the b subunit. Shown are the approximate
 locations of the expressed b subunit mutants constructed and studied in our
 laboratory. Red indicates mutants constructed and studied in the present
 chapter. Shown in blue is the mutant created and studied in Chapter 3. The
 purple stars indicate mutants constructed and studied by Stephanie Cole. The
 blue stars indicate mutants constructed and studied by Megan Greenlee. The
 orange stars indicate a select few of the mutants constructed and studied by
 Dr. Deepa Bhatt. The green star designates the region of insertions and
 deletions studied by Dr. Paul Sorgen. The cyan star shows a mutant
 constructed and studied by Andrew Hardy. Growth indicates the ability of the
 strain to compliment the E. coli KM2 (Ab) cell line and grow on minimal
 succinate media. Symbols are as follows: +++, colonies >1.5 mm; -, no
 growth; (+), sporatic colonies from reversions.