difference in the b subunit is clear between the Al l and +11, confirming the expression

of unequal length b subunits.








 b>

 Figue 44. Wsten blt aalyis o cytein muant suunis ofdiferin legth







 Figre4-4WSDSr Biota Readysi gel andthein transerdto a sbnitr oc fellulos memrne in
 border to prob with ati-bantibormd ies. amdspE 9(ay~b),p



 Inte urrnen chpteprt we hae deelpated na set o twelve uc opiero exression




plasmids that encode amino acid substitutions to void the F1Fo ATP synthase complex of

all known reactive thiols as well as generate strategically placed cysteines. Cysteines

were chosen because the thiol side chain is highly reactive and can be modified by

maleimide reagents. The cysteine mutations did not affect enzyme assembly or function.

Plasmids were designed to express a single cysteine at one of two locations in the

6 subunit as well as one position, either above or below the site of insertion or deletion, in

the b subunit (Figure 4-5).

 The idea for establishing a single reactive cysteine in both Fl and Fo is to use them

as targets for labeling with fluorescence compounds. Fl can readily be stripped from Fo,