A1l53aend-his














 barg364ile-V5













Figure 3-11i. Model of F1Fo ATP synthase incorporated with complementing defective b
 subunits. The barg364ile Subunit was expressed with a V5 epitope tag at its
 carboxyl terminus (represented by the "S"-shaped red line) and the bl534end
 subunit was expressed with a histidine tag at its amino terminus (represented
 by the orange curved line). Complexes incorporated with a b subunit
 heterodimer resulted in a functional enzyme complex. The barg364ile-V5 Subunit
 provides an intact Fl binding domain for interaction with the 6 subunit, and
 the bl534end-his subunit carries out the interaction with the a subunit.