ATP


Ab,
A15l 34end- his
barg364il e-V5~
bargesoglu-v5


bA15s3wend- his/bar g3 eglu-vs


bvt-his/b,_,s


2 min


Figure 3-9. ATP-driven energization of membrane vesicles incorporated with F,Fo ATP
 synthase containing complementing defective b subunits. Membrane
 preparation and ATP-driven proton pumping was accomplished as described
 in Materials and Methods. The samples for each trace have been labeled
 according to the b subunit mutation and the epitope tag, so the strains used as
 the sources of the samples were as follows: Ab, KM2/pBR322; bwt,
 KM2/pKAM14; bl534end-his, KM2/pTAM51;, barg364ile-V5, KM2/pTAM53;
 barg364glu-V5, KM2/pTAM54; bwt-his/bwt-vs, KM2/pTAM3 7/pTAM46; bl534end-
 his/barg364ile-V5, KM2/pTAM51/pTAM53; bl534end-his/barg364glu-V5,
 KM2/pTAM51l/pTAM54.