b+124-130-his



















bd-his


bet-vs/be-his
bsytS/b+1 24-1 so-his
bef-vs


Figure 3-7. ATP-driven energization of membrane vesicles incorporated with a defective
 b+124-130 Subunit mutation. Membrane preparation and ATP-driven proton
 pumping was accomplished as described in Materials and Methods. The
 samples for each trace have been labeled according to the b subunit mutation
 and the epitope tag, so the strains used as the sources of the samples were as
 follows: Ab, KM2/pBR322; bwt, KM2/pKAM14; bwt-vs, KM2/pTAM46; bwt-
 his, KM2/pTAM37; b+124-130-his, KM2/pTAM38; bwt-his/bwt-vs,
 KM2/pTAM3 7/pTAM46; bwt-vs/b+124-130-his, KM2/pTAM46/pTAM3 8.


AT P


2 min