b subunits, b+7-his and bA7-HA, did not dimerize. Similar experiments were performed with

a seven amino acid size difference as opposed to the fourteen amino acid difference just

attempted (data not shown). The immunoblot analysis gave identical results to the

previous data, b+7-his and bwt-HA dimerization was not detected, suggesting the two

different b subunits had incorporated into F1Fo ATP synthase in a segregated manner.

However, further experimentation utilizing this experimental approach indicated that

dimerization was not detected between two wild type length b subunits, bwt-his and bwt-HA,

 Mern brane prep. Ni-Resin Purified




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