mL, milliliter MOPS, 3- [N-morpholino]propanesulfonic acid NADH, P-nicotineamide adenine dinucleotide, reduced form NFDM, nonfat dry milk Ni-CAM, high capacity nickel chelate affinity matrix NMR, nuclear magnetic resonance spectroscopy PAGE, polyacrylamide gel electrophoresis PBS, phosphate-buffered saline PBST, phosphate-buffered saline supplemented with 0.1% tween20 PCR, polymerase chain reaction Pi, inorganic phosphate P/O, number of ATP's made per 2 e- transferred to oxygen PVDF, polyvinylidene fluoride rms, root mean square ser, serine SDS, sodium dodecyl sulfate lauryll sulfate) TID, 3 -(trifluoromethyl)-3 -(m- [125I]i Odophenyl)di azirine TBS, tris-buffered saline thr, threonine TTBS, tris-buffered saline supplemented with 0. 1% tween20 TD, taurodeoxycholate TE, tris[hydoxymethyl]aminomethane, ethylenediaminetetraacetic acid buffer, pH 8.0 Thl, tri s[hy doxym ethyl iami nom ethane, magne sium sulfate buffer, pH 7.5 XV111