LIST OF FIGURES


Figure pg

1-1. Timeline of developing views of F1Fo ATP synthase............... ...............5

1-2. Space-filling structural model of Escherichia coli F1Fo ATP synthase............._.._. .....7

1-3. Structure of the a subunit of E coli F1Fo ATP synthase............... ...............19

1-4. Controversial models of the a subunit topology ....._.._.. .... ... .__. ........_.......29

1-5. Amino acid sequence of the E. coli F1Fo ATP synthase b subunit............_..._... ..........3 8

1-6. Gross structure of the E. coli F1Fo ATP synthase and the domains of the b
 subunit ........._..._... ...............40.._.._.. .....

1-7. Model for F1Fo ATP synthase peripheral stalk orientation dependent upon the
 direction of rotation during ATP synthesis or hydrolysis ..........._.._ ........._..__....47

1-8. Speculative models for the b-like subunits ........._..._... ........._..._...55......_. .

1-9. Model of proton translocation and torque generation in Fo........ .............. .59

1-10. The binding change mechanism .............. ...............63....

2-1. Oligonucleotides for epitope tags and mutagenesis of uncF(b) ................ ...............74

2-2. Construction of the single transcript expression system .............. ....................7

2-3. Histidine and HA-epitope-tagged b subunit expression system ............... ..............89

2-4. Western blot analysis s of hi stidine and HA-epitope tagged b subunits .....................92

2-5. Investigation of detergent solubilization of F1Fo ATP synthase complexes .............94

2-6. Ni-resin purification of F 1Fo, expressing different length b subunits, treated with
 the cross-linker BS3 ........._.__ ..... ._._ ...............95...

2-7. Ni-resin purification of histidine and HA-epitope tagged F1Fo treated with the
 cross-linker BS3 ................ ...............96........... ....

2-8. Histidine and V5-epitope-tagged b subunit expression system ........._...... ..............98