Reddy, 1998) and to increase after the drying of wet soils (Cabrera 1993; Bridgham et al., 1998). N ammonification rates were specifically found to be 2-3 times greater in drained wetland soils (White and Reddy, 2001; Venterlink et al., 2002). Benthic ammonification rates have also been shown to be approximately 2 times greater in Everglades sediment cores than the corresponding deeper layers (White and Reddy, 2001). The objectives of this study were to determine; (1) the response of enzyme activities to a controlled water level decrease in soil cores extracted from a marl dominated wetland (Taylor Slough in Everglades National Park (ENP-TS)) and a peat dominated wetland (Water Conservation Area 3A (WCA-3A)); (2) the differences in response to drained and flooded conditions between the two sites; and (3) any effects that an extended laboratory incubation has on enzyme activities. Materials and Methods Site Description The Florida Everglades is an oligotrophic system with reference surface water total phosphorus (TP) levels averaging less than 10 pg L-1 throughout the interior of the marsh (McCormick et al., 2003). Field study sites were located within the interior of WCA-3A and ENP-TS. WCA-3A encompasses 2,012 km2 and is predominantly a vast peat sawgrass marsh interspersed with sloughs, tree islands, and wet prairies. It is the only area not completely enclosed by levees. The highest annual mean surface water TP levels in the inflow and interior marsh were 67.3 and 20.3 pg L-1, respectively for 1978-2000 (Newman et al., 2002). The vegetation at the sampling site is generally composed of water lilies, spikerush, and periphyton assemblages.