the bilogarithmic relationship between metabolic rate and body mass for this population
of lungfish across a broad size range of 14 juvenile fish. Repeated measures analysis of
variance was used to calculate the adjusted mean resting rate of metabolism at each water
temperature. Following Potvin et al. (1990), Mauchly's criterion was used to test for the
compound symmetry of the variance-covariance matrix. The Qio value relating RRM to
water temperature over a 100C range was calculated using the equation: Qio=(K2/K1)(10/t2-
tl), where K2 and Ki are metabolic rates at temperatures t2 and ti. The determination of
the Qio value indicates an adjustment in RRM to temperature changes across a known
temperature gradient (Winberg 1956) and was determined for Protopterus aethiopicus
over the range of 20 'C to 30 OC. All errors reported are standard errors.
Results
Protopterus aethiopicus showed a positive bilogarithmic relationship between the
rate of oxygen consumption and body mass (range=44 to 222 g) at all three water
temperatures (20 'C: r2=0.798, P=0.016; 25 'C: r2=0.847 P=0.009; 30 'C: r2=0.862,
P=0.007, Figure 3-1). Because of the relatively small sample size for which we could
repeatedly measure metabolic rates across a temperature gradient, we used the slope of
the bilogarithmic relationship described in Chapter 2 (0.78) to derive adjusted mean
resting metabolic rates at each of the three temperatures. Adjusted mean RRM ranged
from 0.00833 mg 02 hf1 at 200C to 0.01567 mg 02 hf1 at 250C and 0.02617 mg 02 hr1 at
30C (adjusted to a mean body mass of 110.5 g, Fig 3-2).
The mean RRM at each temperature interval was significantly different from the
preceding interval (20 'C vs 25 'C: p=0.006, and 25 'C vs 30 'C: p=0.019, repeated
contrasts, Fig. 3-2).