The population is the collection of response variable measurements on all experimental units in the universe. The collection of observations of IGF-1 receptors on each gingival tissue sample make up the population. The U-sample is the collection of experimental units included in the study and the P-sample is the collection of response variable measurements from the U-sample. In this report the U-Sample is the collection of gingival tissue samples in this study and the P-Sample is the expression of IGF-1 receptors on the human gingival tissue samples collected. This is an observational study as it contains only observational factors. The response variable is ordinal as it can be ranked subjectively (grade I, II, or III). Ordinal response variables are the only type of response variable which do not have common distributions to determine mathematically. Hence, they are referred to as distribution free. The statistical analysis of ordinal response variables has historically not involved parameters. This has led to the development of a group on analyses generally referred to as nonparametric because they are not based on population parameters. Thereby validating the use of analysis for linear trend and proportions.