Table 3-2: Percent Distribution % of Grade I % of Grade II % of Grade III Diabetic 2/8 = 25% 2/8 = 25% 4/8 = 50% Non-diabetic 6/14 = 42.9% 3/14 = 21.4 % 5/14 = 28.5% Chi square for linear trend: 0.6516 p value: 0.4196 Grading Distribution 60.0 50.0 40.0 30.0 20.0 10.0 0.0 50.0 42.9 25.0 * Diabetic * Non-Diabetic Grade I Grade II Grade III Figure 3-3: Grading Distribution The universe of a study is the total collection of objects that are of interest in the project. For this investigation the universe is the collection of gingival tissue samples or more practically gingival tissue in adults. Each gingival tissue sample is the experimental unit. A convenience sample was used as the experimental units were chosen from those available in the local setting and agreed to participate. The response variable is the observation or outcome measurement that records the state of the physical phenomenon being studied. The response variable for this study is the observation of IGF-1 receptors (ranked grade I through III subjectively).