CHAPTER 2
 EXPERIMENTAL

 Chemicals

 All Chemicals were purchased from the Aldrich Chemical Co., unless otherwise

noted. Solvents were used as received from Fisher Scientific, unless it is stated that dried

solvents were used. Dried solvents were obtained from the Aldrich keg system and dried

over A1203. Triethylamine was purchased from Aldrich and purified by distillation over

CaH2. The second-generation Grubbs' ruthenium catalyst (Figure 1) (tricyclohexyl

phosphine[1,3-bis(2,4,6-trimethylphenyl)-4,5-dihydroimidazol-2-ylidene][benzylidene]

ruthenium (IV) dichloride) was used exclusively and was synthesized as described

previously by Grubbs' et al.14-17 4-(dimethylamino)pyridine (DMAP) was used as

received from Lancaster. D-(+)-2-amino-3-phenyl-1-propanol (Phenylalaninol) was used

as received from Fisher Scientific Co. The enzymes were used as received from Sigma

Aldrich chemical company after storage according to specifications.

 Instrumentation

 All 1H NMR (300 MHz) and 13C NMR (75 MHz) spectra were recorded on a

Varian Associates Gemini 300, Varian Associates VXR 300, or a Varian Associates

Mercury 300 spectrometer. All chemical shifts were referenced to TMS (0.00 ppm) as an

internal standard for 1H NMR and to CDC13 (77.23 ppm) for 13C NMR.

 Gel permeation chromatography (GPC) of the unsaturated ADMET polymers was

performed using a Waters Associates GPCV2000 liquid chromotography system with its

internal differential refractive index detector (DRI), internal differential viscosity detector