2     3     4      5     6     7


25-0 20-r 15
10l Op


10.6


Figure 4-2. Purification of the polyhedral organelles of S. enterica. Lanes 2-7 contain
          20 tg of protein. Lane 1, molecular mass markers; lane 2, crude lysate; lane
          3, detergent-treated crude lysate; lane 4, 12,000 x g supernatant; lane 5, 49,000 x g pellet; lane 6, second 12,000 x g supernatant; lane 7, density
          gradient purified polyhedral organelles. Molecular masses in kilodaltons are
          shown at the left.


1507550
37-


M       e, 58.6 ,--- 51.9
    A042.6
*-- 38.5
         29.5
            2 7. 2
anew  17.6
          11.1