Figure 4-1. Electron micrographs of polyhedral organelles purified from S. enterica. A. Low-magnification (53,OOOX) electron micrograph of polyhedral organelles. B. High-magnification 122,OOOX) micrograph of polyhedral organelles. The polyhedra are approximately 100-150 nm in diameter and are composed of an interior filled with heterogeneous particles surrounded by a 3-4 nm thick protein shell. The bars in the lower right-hand corners are 100 nm in length. Prior to electron microscopy, organelles were fixed with Trump's reagent and then negatively stained with uranyl-acetate.