it - . S 2"'-. F, i F'P rt : -t A. -- D Figure 3-4. Localization of diol dehydratase and the PduA protein by immunoelectron microscopy. Cells were grown on succinate minimal medium supplemented with PD to induce expression of thepdu operon. For each panel the arrows point to gold particles that indicate the location of either diol dehydratase or the PduA protein. The strain and protein labeled in each panel are as follows: A, S. enterica, diol dehydratase; B, BE182 (ApduA), diol dehydratase; C, S. enterica, PduA; D, BE182 (ApduA), PduA. Bars in lower right-hand corners are 100 nm in length. -