67 ,.~. . . '. . ".' ".:. :. . . . ' . . - ." AC Figure 3-2. Overexpression of the HiS6-PduA protein in E. co/i strain BE230. Cells were grown to mid-log phase and induced with IPTG as described in the text. Plates were purified as described in the materials and methods section. Panel A and B, longitudinal and cross-section of strain BE230 overexpressing the His6-PduA protein; Panel C, purified PduA plates. Bars in lower right-hand corners are 100 nm in length.