66



 kDa       1    2    3   4    5    6

200-


116.2597.4
   66.2

   45 

   31 "now.

   14.4
   21.5
   165 - ;so


4-11.8 kDa


Figure 3-1. Overexpression and purification of the His6-PduA protein. Lane 1,
          molecular mass markers; lane 2, uninduced boiled cell lysate; lane 3, induced boiled cell lysate; lane 4, soluble fraction; lane 5, inclusion body prep; lane 6, combined fractions 12 tol 5 from Ni2+ column elution. Molecular masses in
          kiloDaltons are shown at the left.