Figure 2-7. Packaging of diol dehydratase. The location of diol dehydratase was examined in strain GH1 10, a diol dehydratase mutant that carries plasmid pXY39 encoding the three subunits for diol dehydratase using immunolabeling with anti-diol dehydratase antiserum. The location of diol dehydratase was examined in strains where diol dehydratase expression had been induced both prior to and subsequent to polyhedral organelle formation. Panel A, diol dehydratase expression induced before formation of the polyhedral organelles; Panel B, diol dehydratase expression induced subsequent to organelle formation. Panel C, diol dehydratase mutant (BE87). Gold particles (black dots) indicate the position of diol dehydratase and arrowheads indicate the position of the polyhedral organelles. The bar in the lower right-hand corner is 100 nm in length.