determined using similar methods by Lydia Jorge, Elizabeth Mihalcik, and Dr. Thomas Bobik.


Table 2-1. Bacterial strains Species           Strain
E. coli


Genotype


                   DH5c       F- - endA hsdR]7 relA1 supE44 thi-1 recA
                              gyrA96 A(laclZYA-argF) U69 (480dlacZAM]5) HB101      F- A(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacYl
                              A(mcrC-mrr) rpsL20 xyl-5 mtl-i recA13 S17.1 Apir recA (RP4-2-Tc::Mu) Apir GH1        HB 101/pGH1 (contains ApduCDE insert, AmpR)
                   GH13       S17.1 Apir/pCVD442 (contains ApduCDE insert
                              from GH1, AmpR)
                   RT1679     DH5a/pVJ70 pduF'ABCDEG (AmpR)
 S. enterica serovar          Wild type
 Typhimurium LT2
                   TR6579     metA22 metE551 trpD ilv-452 hsdLT6 hsdSA29
                              HsdB- strA120 GalE- Leu- ProBE33                          pduA::T-POP 11a
                   BE34       pduC::T-POP21a
                   BE35       pduC::T-POP23a
                   BE37       pduA ::T-POP43a
                   BE38       pduB::T-POP78a
                   BE47       thr-480: :Tnl0dCam
                   BE79       ApduCDE thr-480::Tnl0OdCam (precise nonpolar
                              deletion of pduCDE)a BE87                         ApduCDE
                   BE 13      MetE205 ara-9 A299/plac22 (AmpR)
                   BE235      (pdul2:.:MudJ)
                   GH4        pduA ::TPOP11/pTA749 (pduA gene under place
                              control, AmpR)
                   GH9        TR6579/pGH1 (pduB under plac control, AmpR)
                   GH110      DELpduCDE/pXY39 (pduCDE under place control,
                              AmpR)
aSee Materials and Methods.

Genetic Techniques

     Transductional crosses were accomplished using the high-frequency generalized

transducing phage P22HT105/1 int-201 (Schmieger 1971). Transductants were single

colony purified on non-selective green indicator plates (Chan et al. 1972). Cross-