DNase DTT E. coli

EDTA EM

G

HO-B12 hr

IPG IPTG Kan kDa



LB

M

MALDI-TOF MeOH MES

min mM MOPS MS NaCl


deoxyribonuclease dithiothreitol Esherichia coli

ethylenediamine tetraacetate electron microscopy guanosine hydroxy-B12, hydroxycobalamin hour

immobilized pH gradient isopropyl-P-D-thiogalactopyranoside kanamycin kiloDaltons wavelength (nm) Luria-Bertani medium molar concentration matrix assisted laser desorption/ionization time of flight methanol

morpholino ethanesulfonic acid minutes

millimolar morpholino propanesulfonic acid mass spectrometry sodium chloride