sectioned into two columns, on both sides of the hole, measuring 50mm each. From there, the rest of the columns are 100mm in width covering the rest of the bone. The grid also has three rows separating periosteal, center, and endosteal sections.
7) Area per section
a) Number of squares (measuring 10,000 [tmA 2) covering bone are counted
b) This number is multiplied by 10,000 giving the area of bone for each
quadrant
8) Osteon count
a) Haversian canals are marked red with marker
b) Count number of red dots in each quadrant
9) Resorption space area
a) Resorption spaces are marked blue
b) In each quadrant, the number of small boxes (400 [tmA 2) colored
blue are counted
c) The number is then multiplied by 400 giving the total resorption
space area for that quadrant
10) Volkmann' s canal length
a) Volkmann's canals are marked yellow
b) Number of small boxes (400 [tmA 2) colored yellow are counted
c) This number is then multiplied by 20 to give the total length of the canals for the
individual quadrant
11) The data collected for steps 8-10 is then entered into an Excel spreadsheet. These are entered into the appropriate column and then divided by the area of bone found in that particular quadrant on the grid.
12) Preparing mother image for osteon diameter measurements
a) Open mother image (6165x822) in Paint Shop
a. Locate where the lamellar layer ends, or the drill hole start, for the medial
and lateral sides
b) Record these numbers, in pixels, in orange cells on specific chart. The yellow
cells are the location, in pixels, of the scan lines for osteon diameter
measurements. The two cells under the yellow cells are the locations of the
boundary lines.
c) The numbers in the yellow and white cells must be entered in Matlab
d) Run the program
13) Labeling the sections
a) Open mother image, now containing scan lines and boundary lines, in
Paint Shop.
b) Label each section with a letter following the below lettering system: