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METS:mets LABEL CONTRIBUTION OF MODULUS TO THE CONTACT GUIDANCE ENDOTHELIAL CELLS ON MICROTEXTURED SILOXANE ELASTOMERS OBJID UFE0000364 TYPE monograph xmlns:METS http:www.loc.govMETS xmlns:daitss http:www.fcla.edudlsmddaitss xmlns:dc http:purl.orgdcelements1.1 xmlns:mods http:www.loc.govmodsv3 xmlns:palmm http:www.fcla.edudlsmdpalmm xmlns:rightsmd http:www.fcla.edudlsmdrightsmd xmlns:techmd http:www.fcla.edudlsmdtechmd xmlns:xlink http:www.w3.org1999xlink xmlns:xsi http:www.w3.org2001XMLSchema-instance xsi:schemaLocation ..E20051012_AAAABMlinks_20051012234823www.loc.govstandardsmetsmets_LOC.xsd ..E20051012_AAAABMlinks_20051012234823dublincore.orgschemasxmlssimpledc20021212_LOC.xsd ..E20051013_AAAAAKlinks_20051013064438www.loc.govstandardsmodsv3mods-3-0_LOC.xsd ..E20051012_AAAABMlinks_20051012234823www.fcla.edudlsmdtechmd_LOC.xsd ..E20060621_AAAELKlinks_20060621194313www.fcla.edudlsmdpalmm_LOC.xsd ..E20051012_AAAABMlinks_20051012234823www.fcla.edudlsmdrightsmd_LOC.xsd ..E20060502_AAACYYlinks_20060502001940www.fcla.edudlsmddaitssdaitss_LOC.xsd
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mods:title CONTRIBUTION OF MODULUS TO THE CONTACT GUIDANCE OF ENDOTHELIAL CELLS ON MICROTEXTURED SILOXANE ELASTOMERS
mods:name
mods:namePart WILKERSON, WADE R
mods:role
mods:roleTerm type text creator
Anthony B. Brennan
contributor
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mods:publisher University of Florida
mods:dateIssued 2001
20011215
mods:language
mods:languageTerm English
mods:abstract Contact guidance is a term used to describe a material's ability to direct the alignment and growth patterns of biological cells or tissue. It has long been understood that the surface a cell grows on impacts its size, shape, and metabolism. Typically, when cells are exposed to mechanical features such as ridges and grooves, the cells align and travel along the length. Surface chemistry plays a significant role in the attachment of cells to a substrate and in their movement on that surface.
The objective of this study was to study the effect of modulus as well as surface texture dimensions on vascular endothelial cells (ECs). To examine the effects of contact guidance on silicone elastomers, microtextured substrates were produced with reproducible and well-defined surfaces. Ridges of 10,000 �m length were fabricated at 3 different widths: 5 �m, 10 �m, and 20 �m, separated by 5 �m wide grooves to determine the effect of separation of features on the alignment of porcine vascular endothelial cells (PVECs). Two depths were examined: 5 �m and 1.5 �m.
Elastomer samples were examined with contact angles to determine their relative wettability and surface free energy. Formulations of elastomer with both functionalized and non-functionalized PDMS oligomer additives to alter the modulus were examined by contact angle, with no significant difference in surface energy. Surfaces were treated with fibronectin and radiofrequency glow discharge plasma in argon for 5 minutes at 50 W. Both treatments significantly increased the hydrophilicity after treatment, as measured by captive bubble contact angles. Dynamic contact angle analysis of textured surfaces showed a difference in smooth and textured areas as well.
Contact guidance of PVECs on textured silicone elastomers was measured by the nuclear form factor, in which the log of the ratio of nuclear length to width was presented. Results demonstrated that as the ridge width decreased from 20 �m to 5 �m contact guidance increased, as well as when the depth of the grooves increased from 1.5 �m to 5 �m. Data analysis showed that the groove depth was the most important factor in nuclear alignment. Contact guidance on fibronectin-coated elastomers was examined to determine the effect of modulus. It was expected that higher modulus materials would increase the effect of contact guidance. Elastic modulus on 4 elastomers was measured by tensile tests and resulted in a range of values from 0.3 MPa to 2.3 MPa. There was no significant difference in the contact guidance on the deep 5 �m grooves with varying modulus. The 1.5 �m deep grooves showed a significant increase in the alignment of cells to the groove in the highest modulus material compared to the lowest modulus material for the 5 �m and 10 �m wide ridges. The conclusion to be taken from these data is that modulus does seem to play a role in the determination of contact guidance, but other factors such as groove width and especially depth are more significant.
mods:subject
mods:topic contact guidance, microtexture, endothelial cell, PDMS, silicone, biomaterial
mods:accessCondition useAndReproduction Public
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