Image Processing Technique






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Figure 4.2 Steps in image processing technique of hematoxylin stained nuclei. The
original picture (A) is contrasted (B), rotated to align the textures (C), and then measured
for length (D) and width (not shown). The reported value is log(Length/Width)




 The benefit of the vertical alignment is to improve the efficiency of measuring the

nuclei. The procedure introduced by Dunn and Heathl10 requires the measurement of the

length and width of nucleus at its widest point, where the length measured is parallel to