ultra-high purity DI water. Bovine plasma fibronectin was received from Sigma (cat # F-

4759, 2 mg) as a lyophilized powder.


Table 3.2 PDMS additives to silicone elastomer system
Name Molecular Structure Viscosity MW Catalog
 (cSt) (g/mol) Number
Vinyl terminated CH3 CH3 CH3 2-3 550 DMS-V03
PDMS H2C=C-Si-d-Si- -Si-c=cH2 1000 28,000 DMS-V31
 H I n I H
 CH3 CH3 CH3

Vinyltris(trimethyl- / N/A 322.70 SIV-9300
siloxy)silane s---o s'

 Si- -



Trimethylsiloxy CH3 CH3 CH3 50 3,780 DMS-T15
terminated PDMS H3C-- i-O -- I--CH3 5000 49,350 DMS-T35
 I L I J n I
 CH3 CH3 CH3
Tris(trimethylsiloxy) N/A 296.66 SIT8721.0
silane -o --


 SI--
 s-Sl--/


 Methods

Elastomer Preparation

 Unmodified elastomer substrates were produced according to manufacturer

instructions. In a tri-cornered polypropylene beaker, resin and curing agent were added

in a 100:10 resin to curing agent ratio by weight. The two components were mixed with

a metal spatula until well incorporated. During the mixing process, many air bubbles

were trapped in the resin, and so the mixture was degassed under vacuum for 10-15