3.12 3-D images of 5 .im deep etched silicon wafers at each width spacing, taken with
 optical profilom eter ........ .... ...................... .... .............. .. ........ .... 54

3.13 3-D images of 5% LMW elastomer copies (A, B) of a 1.5 tmm wafer (C, D).........55

4.1 Representation of the values measured in the nuclear form factor.........................63

4.2 Steps in image processing technique of hematoxylin stained nuclei. The original
 picture (A) is contrasted (B), rotated to align the textures (C), and then measured for
 length (D) and width (not shown) ............................ .. .... .. .................64

4.3 Main Effects Plot of the data means of fibronectin coated unmodified elastomers at
 various ridge widths (Feature) and groove depths (Depth) ....................................67

4.4 Interaction plot representing the change in contact guidance with depth and feature
 width for fibronectin coated silicone elastomer.................................................. 68

4.5 Main Effects Plot of the data means for plasma treated unmodified elastomers......69

4.6 Interaction plot representing the change in contact guidance with depth and feature
 width for plasma treated silicone elastomer.............. ...............................................70

4.7 Comparison of surface treatments on textured unmodified silicone elastomer by
 ridge width and groove depth (error bars represent standard error of mean) ...........71

4.8 PVECs grown on untextured fibronectin coated LMW sample stained with
 h em ato x y lin .................................................... ................ 7 2

4.9 PVECs grown on 5 [tm spacing 1.5 [tm deep untreated LMW stained with
 h em ato x y lin .................................................... ................ 7 2

4.10 Main effects plot comparing log (L/W) to feature width, feature depth, and
 material used on fibronectin-coated elastomers..............................................73

4.11 Interaction plot representing the change in factors of PVECs grown on fibronectin
 coated m materials. .....................................................................73

4.12 Comparison of materials on textured unmodified silicone elastomer by ridge width
 on 5 tm deep grooves (error bars represent s.e.m.).............................. ........ 75

4.13 Comparison of materials on textured unmodified silicone elastomer by ridge width
 on 5 tm deep grooves (error bars represent s.e.m.).............................. ........ 75

4.14 Comparison of ridge width and groove depth to the high modulus material (LMW)
 and the low modulus material (Tris) (error bars represent s.e.m.)....................76