76 desorbed PCP due to biodegradation, therefore these two sets of results define an upper limit and a lower limit for the degradation rate constants and half-lives for each sample. 5.4.1 Nutrient Requirement. The purpose of this test was to determine how much, if any, nutrient is needed for the biodegradation of phenolic compounds in this particular type of soil. Background analyses indicated that there was enough soluble phosphorus in the soil but the nitrogen concentration was at near the limit of detection (0.01 mg/) Therefore the effects of nitrogen content (at three levels) were tested. Biological degradation was confirmed by comparing samples with controls, and was the main contributor to the decrease of phenol concentrations. The result showed no differences among these treatments as presented in Figures 5-8 and 5-9, indicating that at least part of the phenol was assimilated for energy but not for growth. 5.4.2 Single Compound Biodegradation. Batch biodegradation experiments for phenolic compounds were performed. Treatments with indigenous soil bacteria and amended with municipal wastewater sludge were included. The apparent degradation rate constants were calculated based on first order reaction kinetics. Phenol degraded rather quickly, with average half-lives ranging from 9 hours for the C =5ppm group to 15 hours for 0